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Sequences regulating temporal poly(A) site switching in the adenovirus major late transcription unit.

TitleSequences regulating temporal poly(A) site switching in the adenovirus major late transcription unit.
Publication TypeJournal Article
Year of Publication1991
AuthorsDeZazzo JD, Falck-Pedersen E, Imperiale MJ
JournalMol Cell Biol
Volume11
Issue12
Pagination5977-84
Date Published1991 Dec
ISSN0270-7306
KeywordsAdenoviruses, Human, Cell Line, Humans, Mutagenesis, Poly A, Regulatory Sequences, Nucleic Acid, RNA Processing, Post-Transcriptional, Transcription, Genetic, Transfection, Virus Replication
Abstract

Temporal regulation of poly(A) site choice occurs in an adenovirus recombinant encoding a miniature version of the major late transcription unit with two poly(A) sites, L1 and L3. Using deletion mutagenesis, we have looked directly for cis-acting elements regulating poly(A) site choice in this recombinant. From this work, we draw two main conclusions. First, elements other than the AAUAAA and downstream sequences of the L1 poly(A) site are required for temporal regulation of poly(A) site choice during infection. Second, these regions function in two distinct modes during infection. The two regions enhance selection of the L1 poly(A) site in an additive manner during an early infection, but deletion of either element abolishes the switch in poly(A) site choice during a late infection. This work documents the first example of a regulatory element downstream of a core poly(A) region.

DOI10.1128/mcb.11.12.5977
Alternate JournalMol Cell Biol
PubMed ID1944273
PubMed Central IDPMC361759
Grant ListGM34902 / GM / NIGMS NIH HHS / United States
GM41967 / GM / NIGMS NIH HHS / United States

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